Tris-Glycine SDS Running Buffer

Author

Affiliation

Pranav Mishra

Rush University

Overview

Tris-Gycine Transfer Buffer has a pH of 8.3. Protocol adapted from Abcam and ThermoFicher^{1, 2}

Uses

Running buffer for Western Blot

Components

• Tris Base
• Glycine
• \(HCl\)
• \(diH_2O\)

Protocol

10x Stock

1. Add 700 mL of \(diH_2O\) to a beaker
2. Dissolve 29 g of Tris base, 144 g of Glycine, and 10 g of SDS
3. Adjust pH using \(HCl\), as needed
4. Add \(diH_2O\) until the total volume reaches 1 L of solution

Concentration of 1x Tris-Glycine SDS 25 mM Tris base, 190 mM glycine, 0.1% SDS

Notes

References

1. Transfer and staining of proteins in western blot. Abcam. https://www.abcam.com/protocols/transfer-and-staining-of-proteins-in-western-blot
2. Western Blot Protocols and Recipes. ThermoFicher Scientific. https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-gel-electrophoresis-information/western-blot-protocols.html

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Supplemental

Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals
Tris Base	Ficher	Cat# BP154-1
Glycine	Sigma	Cat# G-7126
SDS - Electrophoresis Grade	Fisher	Cat# BP166-500
HCl	Lab Stock	N/A

Citation

BibTeX citation:

@online{mishra2023,
  author = {Mishra, Pranav},
  title = {Tris-Glycine {SDS} {Running} {Buffer}},
  date = {2023-06-09},
  url = {https://github.com/pranavmishra90/research-reference/protocols/recipes/buffers/tris-glycine-SDS-running-buffer.html},
  langid = {en-US}
}

For attribution, please cite this work as:

Mishra, Pranav. 2023. “Tris-Glycine SDS Running Buffer.” June 9, 2023. https://github.com/pranavmishra90/research-reference/protocols/recipes/buffers/tris-glycine-SDS-running-buffer.html.